Indian Medlars Centre IndMED

Extracted from IndMED

Jeyanthi R; Rao KP.
Biomaterials Laboratory, Central Leather Research Institute, Adyar, Madras 600020, India
Hydrogels for drug delivery: Preparation, characterization and release kinetics
Trends in Biomaterials and artificial organs. 1991 May; 5(2): 72-8

ABSTRACT: Copolymeric hydrogels of collagen and poly (hydroxyethyl methacrylate) poly (HEMA) were prepared using ammonium persulfate-sodium metabisulfite redox initiation technique. The hydrogels were characterized using FT-IR spectroscopy thermogravimetric analysis (TGA) and scanning elect on microscopy (SEM). It was found that hydrogels containing about 5-10 percent (w/w) collagen were stable and resilient. Beyond 10 percent collagen content, the hydrogels were fluid in nature. The FT-IR spectrum of collagenpoly (HEMA) hydrogel indicated the characteristic amide absorption bands of collagen in addition to the ester and hydroxyl bands of poly (HEMA). The TGA curve of the hydrogel showed four stages of thermal degradation. The surface morphology of collagen-poly (HEMA) hydrogel as examined by SEM showed the pore structure and the embedded collagen fibres. Equilibrium swelling behaviour of the hydrogels in water and at different pHs gave interesting results. Three potent anticancer drugs bleomycin (BLM), mitomycin C (MMC) and 5-fluorouracil (5-FU) were entrapped in the hydrogel matrix. The in Vitro release of these drugs from hydrogel matrices in phosphate buffer, pH 7.4 at 37 degree C, was found to be independent of time and the release profiles followed near zero-order kinetics. In the case of 5-FU, upto 98 percent release followed the model for 10 days while in the case of MMC & BLM zero-order behaviour was observed for 12 days with about 52 percent release and 11 days with about 74 percent release respectively. These results clearly indicated that collagen-poly (HEMA) hydrogels can serve as ideal carriers for the controlled release of anticancer drugs.
KEYWORDS:
Hydrogels/CH; Hydrogels/PK; Thermogravimetery; Research; Collagen/PD; Microscopy, Electron, Scanning; Equilibrium; Data Interpretation, Statistical Animal; Cattle; In Vitro
References: 7
Record Identifier: NI203364